Enzymes of Molecular Biology (Methods in Molecular Biology)



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Description
The scientist's understandingo f the cell at the molecularl evel has advancedr apidly over the last twenty years.T his improvedu nderstanding has led to the developmento f many new laboratory methodst hat increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines.

To aid the processo f investigation,t heM ethodsi n Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirementso f the enzymesb eing used.T his information, though usually availablei n the literature,i s often widely disperseda ndf requently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trainede nzymologistsa nd may be unawareo f some of the parameterst hat are important in a specific enzymer eaction.

Content:-
Preface
Contributors
1. Nucleases: An Overview
2. Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)
3. DNA Polymerases (EC 2.7.7.7)
4. Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols
5. Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6)
6. Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase to Synthesize DNA from RNA
7. Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31)
8. Restriction Enzymes
9. DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73)
10. DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3)
11. The BAL 31 Nucleases (EC 3.1.11)
12. Mung-Bean Nuclease 1 (EC 3.1.30.1)
13. RNase A (EC 3.1.27.5)
14. Pronase (EC 3.4.24.4)
15. Proteolytic Enzymes for Peptide Production
16. Proteinase K (EC 3.4.21.14)
17. Carboxypeptidase Y (EC 3.4.16.1)
18. Aminopeptidases: Aminopeptidase M (EC 3.4.11.2), Pyroglutamate Aminopeptidase (EC 3.4.19.3), and Prolidase (EC 3.4.13.9)
19. Alkaline Phosphatase (EC 3.1.3.1)
20. Polynucleotide Kinase (EC 2.7.1.78)
Index

Author Details
"Michael M. Burrell"
AdvancedT echnologies (CambridgeL)i mited,
Cambridge, UK




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